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SEIYU HOSONO, Ph.D.




seiyu_hosono@yahoo.com
 

LABORATORY
Molecular Staging Inc., New Haven, CT
 

CITIZENSHIP    Japanese, U.S. permanent resident (Green Card)
 

EDUCATION
1986-1992
Ph.D., Cell and Molecular Biology/Graduate Group in Biology.
University of Pennsylvania, Philadelphia, PA

1982-1986
B.S., Molecular Genetics/Biology.
University of Rochester, Rochester, NY

1970-1982
St. Mary's International School (Bilingual Education) Tokyo, Japan

  CURRENT POSITION
2000-present     Molecular Staging, Inc., New Haven, CT
2003-present     Whole Genome Amplification Technology Service/Group Leader
2002-2003        Whole Genome Amplification Technology Process Development/Group Leader
2000-2002        Genomics Section/Research Scientist   POST-DOCTORAL EXPERIENCE
1994-1999         Mt. Sinai School of Medicine, New York, N.Y. Dept. of Medicine
P.I. Dr. Jonathan Licht. Project: Studies of the mechanism of the WT1 tumor suppressor gene
activity and role of WT1 gene in renal differentiation program.

1993          University of Texas Medical Branch at Galveston, TX Dept. of Pathology
P.I. Dr. Chiaho Shih. Project: (1) Studies of the etiology of human hepatitis B virus (HBV)-
related hepatocellular carcinoma (HCC); focusing on the mechanism of immunoevasion of
human hepatitis B virus-positive HCC by elimination of HBV e antigen; (2) Studies of the
translation mechanism of HBV polymerase gene; (3) Studies of the mechanism of the natural
occurrence of HBV e antigen negative mutation.

  PRE-DOCTORAL TRAINING Ph.D THESIS
1987-1992     University of Pennsylvania, Philadelphia, PA  Dept. of Biochemistry and Biophysics.
Thesis advisor: Dr. Chiaho Shih. Thesis project: Studies of the etiology of human hepatitis B
virus-related hepatocellular carcinoma (HCC); focusing on the role of p53 in HCC.   GRADUATE LAB ROTATIONS
1987          University of Pennsylvania, Philadelphia, PA Dept. of Biology.
Advisor: Dr. Eric Weinberg. Lab rotation project: Subcloning and deletion analysis of
Sea Urchin Histone H2B gene promoter region; in vitro transcription of the H2B gene promoter
using Sea Urchin nuclear extract.   1986          University of Pennsylvania, Philadelphia, PA Dept. of Biology.
Advisor: Dr. Yoshitaka Suyama. Lab rotation project: Cloning of the telomere sequence of
Tetrahymena mitochondrial DNA.   UNDERGRADUATE LAB EXPERIENCE
1985-1986    University of Rochester, Rochester, NY Dept. of Radiation Biology and Biophysics.
Advisor: Dr. Carol Moore. Undergraduate independent study project: Genetic mapping of gene
conferring resistance to anti-cancer drug, Bleomycin, in Saccharomyces cerevisiae.   SKILLS AND HOBBIES
Languages: Japanese and English - fluent in oral and written. Computer skill: Competent in a variety of software packages (IBM, Macintosh)-Word Processing, Spreadsheets, Database, Power Point, Computer/Internet (writing HTML script), Painting, Antique Collecting, Reading, Acting, Assistant Research and Production of a TV program, Filmmaking, and Baseball (managing a team and playing).   PROFESSIONAL MEMBERSHIP AND AWARDS
1986-1989    Teaching Assistant Fellowship, The University of Pennsylvania, Dept of Biology
 

SCIENTIFIC PUBLICATIONS: ARTICLES

Gross, I., Morrison, D.J., Hyink, D.P., Georgas, K., English M.A., Mericskay, M., Hosono, S., Sassoon, D., Wilson, P.D., Little, M., and Licht, J.D. (2003). The receptor tyrosine kinase regulator sprouty1 is a target of the tumor suppressor WT1 and important for kidney development. J Biol Chem. 2003; August IN PRESS

Alsmadi, O.A., Bornarth, C.J., Song, W., Wisniewski, M., Du J., Brockman, J.P., Faruqi, A.F., Hosono, S., Sun, Z., Du, Y., Wu, X., Egholm, M., Abarzua, P., Lasken, R.S., and Driscoll, M.D. (2003). High accuracy genotyping directly from genomic DNA using a rolling circle amplification based assay. BMC Genomics 2003; 4(21)

Hosono, S., Faruqi, A.F., Dean F.B., Du, Y., Sun, Z., Wu, X., Du, J., Kingsmore, S.F., Egholm, M., and Lasken, R.S. (2003). Unbiased whole-genome amplification directly from clinical samples. Genome Res. 2003; 13(5): 954-964

Hosono, S., Dean F.B., Fang, L., Wu, X., Faruqi, A.F., Bray-Ward, P., Sun, Z., Zong, Q., Du, Y., Du, J., Driscoll, M., Song, W., Kingsmore, S.F., Egholm, M., and Lasken, R.S. (2002). Comprehensive human genome amplification using multiple displacement amplification. Proc. Natl. Acad. Sci. 2002; 99(8): 5261-5266

Faruqi A.F., Hosono, S., Driscoll, M.D., Dean, F.B., Alsmadi, O., Bandaru, R., Kumar, G., Grimwade, B., Zong, Q., Sun, Z., Du, Y., Kingsmore, S., Knott, T., and Lasken, R.S. (2001). High-throughput genotyping of single nucleotide polymorphisms with rolling circle amplification. BMC Genomics 2001; 2(1): 4

Hosono, S., Gross, I., English, M.A., Hajra, K.M., Fearon, E.R. and J.D. Licht (2000). E-cadherin is a WT1 Target Gene. J Biol Chem 275, 10943-53

Hosono, S., Luo, X., Hyink, D.P., Schnapp, L.M., Wilson, P.D., Burrow, C.R., Reddy, J.C., Atweh, G.F. and J. D. Licht (1999). WT1 Expression Induces Features of Renal Epithelial Differentiation in Mesenchymal Fibroblasts. Oncogene 18, 417-427.

Shih, C., Tai, P-C., Whitehead, W., Hosono, S. (Univ. of Texas Medical Branch) and Lee, C-S., Yang, C-S (Natl. Taiwan University) (1996) Hepatitis B and C viruses and liver cancer. Encyclopedia of Cancer. Academic Press Vol.II, 824-834.

Reddy, J.C., Hosono, S., and J.D. Licht (1995) The transcriptional effect of WT1 is modulated by choice of expression vector. J Biol Chem 270, 29976-82

Luo, X-N., Reddy, J.C., Yeyati, P.L., Idris, A.H., Hosono, S., Haber, D.A., Licht, J.D. and G.F. Atweh. (1995) The tumor suppressor gene WT1 inhibits ras-mediated transformation. Oncogene 11, 743-750

Hosono, S., Tai, P-C., Wang, W., Ambrose, M., Hwang, D.G-Y., Yuan, T-T., Peng, B-H., Yang, C-S., Lee, C-S., C. Shih. (1995) Core antigen mutations of human hepatitis B virus in hepatomas accumulate in MHC class II-restricted T cell epitopes. Virology 212, 151-162.

Hosono, S., Chou, M-J., Lee, C-S. and C. Shih. (1993) Infrequent mutation of p53 gene in hepatitis B virus positive primary hepatocellular carcinomas. Oncogene 8, 491-496.

Hosono, S., Lee, C-S., Chou, M-J., Yang, C-S. and C. Shih. (1991) Molecular analysis of the p53 alleles in primary hepatocellular carcinomas and cell lines. Oncogene 6, 237-243.

SCIENTIFIC ABSTRACTS

Hosono, S., Gross, I. and J.D. Licht (1998) WT1 expression induces features of renal epithelial differentiation and induces expression of known and novel target genes in mesenchymal fibroblasts. Cold Spring Harbor Meeting on Cancer Genetics and Tumor Suppressor Genes (selected for poster presentation).

Yuan, T-T., Faruqi, A., Hosono, S., Shih, J. and C. Shih (1993) The mechanism of natural occurrence of HBV precore predominant mutations. Georgetown University Meeting on Molecular Biology of Hepatitis B Virus. (selected for poster presentation).

Faruqi, A., Hosono, S., Roychoudhury, S. and C. Shih (1993) The mechanism of translational control of HBV polymerase. Georgetown University Meeting on Molecular Biology of Hepatitis B Virus. (selected for poster presentation).

Shih, C., Hosono, S., Tai, P-C., Wang, W., Hwang, G., Yuan, T-T., Peng, B.H., Whitehead, W., Yang, C.S. and Lee, C.S. (1994) The existence of escape mutants of end-stage human hepatitis B virus. Institut Pasteur Meeting on Molecular Biology of Hepatitis B Virus.(selected for poster presentation).

Hosono, S., Lee, C-S. and C. Shih (1993) Immunoevasion of human hepatitis B virus-positive hepatocellular carcinoma by elimination of e-antigen. Georgetown University Meeting on Molecular Biology of Hepatitis B Virus. (selected for poster presentation).

Hosono, S., Pei, D. and C. Shih (1990) Molecular analysis of several putative anti-oncogene alleles in primary tumors and cell lines of hepatocellular carcinoma. UC San Diego Meeting on Molecular Biology of Hepatitis B Virus. (selected for oral presentation).

Hosono, S. and C. Shih (1989) Gene amplification and rearrangement of a human T-cell receptor gamma gene in a liver-associated tumor. Cold Spring Harbor Meeting on Molecular Biology of Hepatitis B virus (selected for poster presentation).
 





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